专利名称：Method for the analysis of Vitamin C发明人：Richard T. Tulley申请号：US08/072595申请日：19930604公开号：US05441872A公开日：19950815
摘要：A new enzymatic rate method is disclosed for the analysis of Vitamin C usingascorbate oxidase. Total Vitamin C can be measured in a single analysis, in a procedureamenable to automation. Any dehydroascorbic acid (DHAA) in the sample is reduced toascorbic acid. Then a coupling agent such as o-phenylenediamine (OPDA) is added to thesample, and any nonspecific reactions with, or spectrometric interferences from, othercomponents of the sample are measured, to be blanked out from the final measurement.Then ascorbate oxidase is added, oxidizing ascorbic acid to DHAA; the DHAA then reactswith the OPDA already present from the prior step, and the quinoxaline derivativeproduct may be measured spectrometrically, e.g., by absorbance at 350 nm.
申请人：BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY ANDAGRICULTURAL AND MECHANICAL COLLEGE
代理人：John H. Runnels
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