SPECIFIC AND UNIVERSAL PROBES AND AMPLIFICATION PR

专利名称：SPECIFIC AND UNIVERSAL PROBES AND

AMPLIFICATION PRIMERS TO RAPIDLYDETECT AND IDENTIFY COMMON

BACTERIAL PATHOGENS AND ANTIBIOTICRESISTANCE GENES FROM CLINICALSPECIMENS FOR ROUTINE DIAGNOSIS INMICROBIOLOGY LABORATORIES

发明人：BERGERON, Michel G.,Ouellette, Marc,ROY,

Paul H.

申请号：EP95931109.0申请日：19950912公开号：EP0804616A2公开日：19971105

摘要：The present invention relates to DNA-based methods for universal bacterialdetection, for specific detection of the pneumoniae, Pseudomonas aeruginosa, Proteusmirabilis, Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epiderminis,Enterococcus faecalis, Staphylococcus saprophyticus, Streptococcus pyogenes,Haemophilus influenzae and Moraxella catarrhalis as well as for specific detection ofcommonly encountered and clinically relevant bacterial antibiotic resistance genesdirectly from clinical specimens or, alternatively, from a bacterial colony. The abovebacterial species can account for as much as 80% of bacterial pathogens isolated inroutine microbiology laboratories. The core of this invention consists primarily of theDNA sequences from all species-specific genomic DNA fragments selected by

hybridization from genomic libraries or, alternatively, selected from data banks as well asany oligonucleotide sequences derived from these sequences which can be used asprobes or amplification primers for PCR or any other nucleic acid amplification methods.This invention also includes DNA sequences from the selected clinically relevant antibioticresistance genes.

申请人：BERGERON, Michel G.,Ouellette, Marc,ROY, Paul H.

地址：2069 Brûlard Street, Sillery Quebec G1T 1G2 CA,975 Casot Street Quebec,Quebec G1S 2Y2 CA,28 Charles Garnier, Loretteville Quebec G2A 2X8 CA

国籍：CA,CA,CA

代理机构：Jaunez, Xavier, et al

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