PCR-FREE SAMPLE PREPARATION AND DETECTION SYSTEMS

专利名称：PCR-FREE SAMPLE PREPARATION AND

DETECTION SYSTEMS FOR HIGH SPEEDBIOLOGIC ANALYSIS AND IDENTIFICATION

发明人：Kyle Wisdom Hukari,Jason Andrew Appleton

West,Dustin Li,Brent ColemanSatterfield,Lars L. Majlof

申请号：US12301099申请日：20070516

公开号：US20100216657A1公开日：20100826

专利附图：

摘要：Provided herein are biologic sample preparation and analysis systems that arerapid, portable, robust detection system for multiplexed detection of bio-threats, andwhich can be ruggedized to operate in harsh environments. A new method of detectioncalled Combinatorial Probe Analysis (CPA), which provides an exponential increase indetection reliability, has been incorporated into these systems. This type of analysisgreatly reduces false positives and false negatives; in addition it is reusable and

eliminates special storage requirements for reagents. Specific technical advancements inthe optimization of hybridization assays for nucleic acid detection on porous polymermonoliths (PPM) are also disclosed. Performing rapid and complete solubilization ofviruses, vegetative bacteria and bacterial spores with an ultra high temperature

solubilization protocol is also described. The systems provided herein provides the abilityto perform rapid highly multiplexed analysis of a variety of bioagents, including bacteriaviruses, and protein biotoxins. The systems and assays described herein are performcompletely automated sample preparation and analysis, in a time frame of five minutesor less. The assay is simple in design allowing users in personal protective equipment toeasily operate the system. The disclosed systems are robust, simple to use, and addressthe goals of the first responder community.

申请人：Kyle Wisdom Hukari,Jason Andrew Appleton West,Dustin Li,Brent ColemanSatterfield,Lars L. Majlof

地址：Dublin CA US,Pleasanton CA US,Berkeley CA US,Pleasanton CA US,Saratoga CAUS

国籍：US,US,US,US,US

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