ASSAY FOR THE DETECTION OF GENETIC ABNORMALITIES

专利名称：ASSAY FOR THE DETECTION OF GENETIC

ABNORMALITIES

发明人：MARTINAZZO, Giorgio,BICHI,

Roberta,MARCOLINI, Stanislavo,TURCHETTI,Elisabetta,PELLICCIA, Angela

申请号：EP94930990.0申请日：19941027公开号：EP0725834A1公开日：19960814

摘要：A process for detecting in an analyte target sequences respectively excluding(wild-type) or including a genetic mutation, comprises the steps of: reacting the analyteunder hybridising conditions with (i) first and second oligonucleotide probes and (ii) firstand third oligonucleotide probes, the probes being respectively complementary to first,second and third regions of the target sequences, wherein the first region is on one sideof the mutation, the second and third probes each span the mutation site and arerespectively complementary to the wild-type and mutated regions, and wherein the firstprobe is separable or detectable and the second and third probes are respectivelyseparable or detectable; and, if necessary, amplifying either or each target sequence; andseparating and detecting the resultant hybrids that carry two probes.

申请人：RAGGIO-ITALGENE SpA

地址：Via delle Antille, 29 I-00040 Pomezia IT

国籍：IT

代理机构：Perry, Robert Edward, et al

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